Second Cycle — Public Data

Overview

This archive contains longitudinal data from an N=1 case study documenting a 28-day cyclic hormone protocol following peritoneal flap vaginoplasty. New cycles are added monthly as they complete.

For context on the protocol, methodology, and what this data represents, see secondcycle.io.

Structure

Data is organized by cycle, with each cycle in its own directory:

/cycle1/
/cycle2/
...

Each cycle directory contains some or all of the following files:

  • cycle{N}_README.md — narrative overview, protocol summary, deviations, data quality notes
  • cycle{N}_protocol_log.csv — day-by-day protocol administration
  • cycle{N}_inito.csv — Inito urinary hormone readings
  • cycle{N}_clue_daily.csv — symptom and cycle tracking
  • cycle{N}_wearables.csv — Apple Watch biometrics
  • cycle{N}_serum_labs.csv — serum lab results (when collected)
  • cycle{N}_evvy_microbiome.csv / cycle{N}_evvy_summary.csv — microbiome results (when collected)
  • cycle{N}_data_dictionary_additions.csv — variable definitions new to this cycle

Not every file appears in every cycle. Files are only present when data was collected. The cycle-specific README documents what is and is not included.

Where to Start

For each cycle, start with its README (cycle{N}_README.md). It covers what happened that cycle, what data was collected, any protocol deviations, and known data quality issues.

The data_conventions.md file in the root of this archive describes project-wide conventions: file naming, schema definitions, formatting rules, and column definitions shared across cycles.

Baseline Data

Pre-intervention and washout-period data (Phase A) is included in the baseline/ directory. This covers the period before the cyclic protocol began and establishes reference values.

License

CC-BY 4.0

Contact

Questions about this data can be directed to E via the contact methods listed at secondcycle.io.

Releases

  • Cycle 1 — Cycle 1 (April 1-28, 2026). Four EV injections, vaginal P4 luteal support, daily Inito + wearables.
  • Baseline — Pre-intervention baseline: washout + early Cycle 1 pre-injection. Includes a pre-washout Nov 2025 lab draw.

Files